● Flexible Hybridization Time

Figure 1. Capture Performance at Different Hybridization Times (On-Target Rate)	Figure 2. Capture Performance at Different Hybridization Times (Uniformity)

Prepared libraries were constructed using QuarPrep EZ DNA Library Kit (Dynegene, Cat. # NL1002), with 500 ng prepared libraries input per capture experiment. Different panel sizes (82.35 kb, 598.47 kb, 40 Mb) were used for hybridization experiments following the QuarHyb Super DNA Reagent Kit (Dynegene, Cat.# NC1009) protocol. Results showed that even at 15 minutes of hybridization, the capture performance remained excellent, allowing users to adjust hybridization times flexibly according to their needs.  

Note: "Overnight-QuarHyb DNA" refers to Dynegene's conventional overnight hybridization reagent, QuarHyb DNA Plus 2 Reagent Kit (Dynegene, Cat.# NC1008).  

 

● Compatibility with Various Panels

Figure 3. Capture Performance Across Different Panels (On-Target Rate)	Figure 4. Capture Performance Across Different Panels (Uniformity)

Prepared libraries were constructed using QuarPrep EZ DNA Library Kit (Dynegene, Cat. # NL1002), with 500 ng prepared libraries input per capture experiment. Different panel sizes were tested for 2-hour rapid hybridization with QuarHyb Super DNA Reagent Kit (Dynegene, Cat. # NC1009), alongside overnight hybridization using Dynegene's conventional hybridization reagent, QuarPrep EZ DNA Library Kit  (Dynegene, Cat.# NC1008).  

Results demonstrated comparable data between rapid hybridization with QuarHyb Super DNA Reagent Kit and overnight hybridization with QuarHyb DNA Plus 2 Reagent Kit, achieving high on-target rates and excellent uniformity.  

Note: The sizes of the panels are as follow: Panel 1: 82.35 kb; Panel 2: 121.93 kb; Panel 3: 598.47 kb; Panel 4: 1602.00 kb; Panel 5: 2039.16 kb; Panel 6: 40 Mb. 

 

● Batch-to-Batch Consistency

Figure 5. Capture Performance Across Different Batches (On-Target Rate)	Figure 6. Capture Performance Across Different Batches (Uniformity)

To validate batch consistency, three reagent batches were tested using an 82.35 kb panel according to the QuarHyb Super DNA Reagent Kit (Dynegene, Cat. # NC1009) protocol. Each batch included six replicates under identical conditions.  

Results indicated stable capture performance across different batches, ensuring reproducibility and reliability.