Library output is the similar as the performance of manual operation

 

Figure 1. Comparison of the Library concentration between iQuars 50 and manual operation

The experiment used standard NA12878, Input 100 ng and enzymatic fragmentation for library preparation (QuarPrep EZ DNA Library Kit 1.0, Dynegene, NL1002). QuarXeq Human All Exon Probes 1.0 (Dynegene, NY1001) was used as the panel for capture, and QuarHyb One Reagent Kit (Dynegene, NC1003)as the capture reagent .Automated process uses built-in applications of the instrument. 750 ng of Pre-PCR library per sample was used for single-hybridization. Manual operations were carried out according to the product instructions.

 

 

Library fragment size is stable

 

Library Fragment Size

Figure 2. Library fragment sizes prepared with iQuars 50，CV 1.53%，Peak stability

 

 

Excellent single-hybridization capture data

Figure 3. Compare the capture data between iQuars 50 and manual operation

The experiment used standard NA12878,Input 100 ng and enzymatic fragmentation for library preparation (QuarPrep EZ DNA Library Kit 1.0, Dynegene, NL1002). QuarXeq NCCN Cancer Panel 1.0 (Dynegene, NY1016) was used as the panel for capture, and QuarHyb One Reagent Kit (Dynegene, NC1003)as the capture reagent .Automated process uses built-in applications of the instrument. 750 ng of Pre-PCR library per sample was used for single-hybridization.

Manual operations were carried out according to the product instructions.

 

Stable multiple-hybridization capture data

Figure 4.multiple-hybridization capture data for iQuars 50

The experiment used standard NA12878, Input 100 ng and enzymatic fragmentation for library preparation (QuarPrep EZ DNA Library Kit 1.0, Dynegene, NL1002), QuarXeq Human All Exon Probes 1.0 (Dynegene, NY1001) was used as the panel for capture, and QuarHyb One Reagent Kit (Dynegene, NC1003) as the capture reagent to carry out single-hybridization, 4-mixed-with-1, 6-mixed-with-1, and 8-mixed-with-1 operations, respectively. Automated process uses built-in applications of the instrument. Manual operations were carried out according to the product instructions.