Dynegene’s CRISPR Workflow: Mastering Oligo Pool Synthesis for Precision Genetics

CRISPR-Cas9 has emerged as a cornerstone of modern genetic engineering, with applications spanning therapeutic development, agricultural innovation, and industrial biotechnology. However, the success of CRISPR screens hinges on one critical factor: high-quality oligo pools. These pools, comprising thousands of single-guide RNAs (sgRNAs), enable researchers to target genes at scale—yet traditional synthesis methods struggle to meet the demands of accuracy, throughput, and cost-efficiency required for large-scale studies.

Dynegene Technologies, backed by investors like Volcanics Venture and ByteDance, has positioned itself as a leader in this field. As a prominent Chinese enterprise specializing in ultra-high-throughput next-generation DNA synthesis, Dynegene’s CRISPR workflow integrates proprietary DYHOW technology and high-standard quality control to deliver oligo pools with high synthesis accuracy, aiming to set a new benchmark for precision genetics.

The Challenges of Oligo Pool Synthesis in CRISPR Workflows

1. Scalability vs. Accuracy: A Delicate Balance

CRISPR genome-wide screens require oligo pools containing 10,000–100,000 unique sgRNA sequences. Traditional column-based synthesis methods are often slow, error-prone, and cost-prohibitive for such scales. For example:

• Error rates of 1:500/nt in conventional workflows can compromise screening results.
• Low sequence uniformity may lead to skewed representation in libraries, reducing screening efficacy.

Dynegene’s DYHOW™ platform aims to address these issues by synthesizing 4.35 million oligos per chip, a significant improvement over many traditional methods.

Applications: Accelerating Discovery Across Industries

Drug Development

• Target Identification: Screen thousands of genes in parallel to identify novel therapeutic targets.
• Resistance Mechanisms: Uncover pathways driving drug resistance in oncology and infectious diseases.

Agricultural Biotechnology

• Crop Optimization: Engineer drought-resistant crops using multiplexed CRISPR libraries.
• Livestock Health: Develop disease-resistant livestock strains through precision editing.

Industrial Biomanufacturing

• Enzyme Engineering: Optimize enzymes for biofuel production using saturated mutagenesis libraries.
• Metabolic Pathway Tuning: Enhance microbial strains for chemical synthesis.

Why Dynegene Stands Apart

Proprietary Technology

• DYHOW Synthesis: Offers a maximum length of 230 nt with 4.35 million different custom sequences.

Investor-Backed Innovation

Supported by leading firms like Boyu Capital and MSA Capital, Dynegene has made significant investments in R&D to advance CRISPR workflows.

Conclusion: Partner with Dynegene for Precision

Dynegene’s oligo pools workflow aims to combine scale and accuracy to empower researchers tackling complex genetic challenges.